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non essential amino acids 100  (Thermo Fisher)


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    Structured Review

    Thermo Fisher non essential amino acids 100
    Non Essential Amino Acids 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+essential+amino+acids+100/2+MERCAPTOETHANOL/pmc12859387-92-52-56
    Average 99 stars, based on 1 article reviews
    non essential amino acids 100 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte
    Article Snippet: .. The following reagents and chemicals were purchased from respective suppliers: Dulbecco’s modified Eagle’s medium (1×) (DMEM; Gibco, cat. no. 11965-092), phosphate-buffered saline (PBS; Gibco, cat. no. 10010-023), Minimum Essential Medium Eagle, alpha modification (1×) (α-MEM; Gibco, cat. no. 12571–063 and 32571-036), 2-mercaptoethanol (55 mM; Gibco, cat. no. 21985-023), fetal bovine serum (FBS; Gibco, cat. no. 16000-044), GMEM (1×) (Thermo, cat. no. 11710-035), KSR (15%) (Thermo, cat. no. 10828-028), retinoic acid (100 mM; Sigma, cat. no. R2625), GlutaMax (100×) (Thermo, cat. no. 32571-036), non-essential amino acids (100×) (Thermo, cat. no. 11140-050), sodium pyruvate (Gibco, cat. no. 11360-070), Y27632 (Peprotech, cat. no. 1293823), penicillin/streptomycin/L-glutamine (100×) (Gibco, cat. no. 10378-016), Insulin-Transferrin-Selenium (ITS; Thermo, cat. no. 41400-045), ascorbic acid (150 mM; TCI, cat. no. G0394), ICI182,780 (Tocris, cat. no. 1047), follicle-stimulating hormone (FSH; Sigma, cat. no. F4021), epidermal growth factor (EGF; R&D Systems, cat. no. 2028-EG-200), polyvinylpyrrolidone (PVP; Sigma, cat. no. PVP360), human chorionic gonadotropin (1,200 IU mL −1 ) (hCG; Sigma, cat. no. C8554), collagenase type IV (1 g mL −1 ; Gibco, cat. no. 17104-019), trypsin-EDTA, 0.25% (Gibco, cat. no. 25200-072), TCM 199(Gibco, cat. no. 11150-059), StemPro-34 SFM (1×), liquid (Gibco, cat. no. 10639-011), and hyaluronidase (500 μg ml −1 , TCI, cat. no. H0164), anti-Ddx4 (polyclonal, 1:500; Abcam), anti-GDF9 (polyclonal, 1:500; Invitrogen), anti-α-tubulin (monoclonal, 1:20, Thermo, cat no. 53-4502-82) fluorescently labeled (Alexa Fluor 488 or 568; Molecular Probes, Eugene, OR, USA), DAPI (molecular probes, Thermo). ..

    Article Title: Adsorption properties of biologically active derivatives of quaternary ammonium surfactants and their mixtures at aqueous/air interface. I. Equilibrium surface tension, surfactant aggregation and wettability.
    Article Snippet: The adsorption properties of surfactant mixtures containing two types of quaternary derivatives of lysosomotropic substances: alkyl N,N-dimethylalaninates methobromides and alkyl N,N-dimethylglycinates methobromides were studied.. Quantitative and qualitative description of the adsorption process was carried out on the basis of experimentally obtained equilibrium surface tension isotherms.. The results indicated that most of the systems studied revealed synergistic effect both in adsorption and wetting properties.

    Article Title: Predicting drug-induced slowing of conduction and pro-arrhythmia: identifying the 'bad' sodium current blockers
    Article Snippet: .. The cells were cultured in Iscove's modified Dulbecco's medium (IMDM, Gibco, Invitrogen, Ltd., Paisley, UK), which was supplemented with (amounts indicated hereafter are added to 500 mL IMDM): 50 mL fetal bovine serum (Bio-Whittaker, Walkersville, MD, USA), 5 mL non-essential amino acids 100× (Gibco) and 10 mL hypoxanthine/thymidine supplement 50× (Gibco). .. Immediately before use, 400 μg·mL −1 geneticin (Gibco) was added to the medium.

    Saline:

    Article Title: Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte
    Article Snippet: .. The following reagents and chemicals were purchased from respective suppliers: Dulbecco’s modified Eagle’s medium (1×) (DMEM; Gibco, cat. no. 11965-092), phosphate-buffered saline (PBS; Gibco, cat. no. 10010-023), Minimum Essential Medium Eagle, alpha modification (1×) (α-MEM; Gibco, cat. no. 12571–063 and 32571-036), 2-mercaptoethanol (55 mM; Gibco, cat. no. 21985-023), fetal bovine serum (FBS; Gibco, cat. no. 16000-044), GMEM (1×) (Thermo, cat. no. 11710-035), KSR (15%) (Thermo, cat. no. 10828-028), retinoic acid (100 mM; Sigma, cat. no. R2625), GlutaMax (100×) (Thermo, cat. no. 32571-036), non-essential amino acids (100×) (Thermo, cat. no. 11140-050), sodium pyruvate (Gibco, cat. no. 11360-070), Y27632 (Peprotech, cat. no. 1293823), penicillin/streptomycin/L-glutamine (100×) (Gibco, cat. no. 10378-016), Insulin-Transferrin-Selenium (ITS; Thermo, cat. no. 41400-045), ascorbic acid (150 mM; TCI, cat. no. G0394), ICI182,780 (Tocris, cat. no. 1047), follicle-stimulating hormone (FSH; Sigma, cat. no. F4021), epidermal growth factor (EGF; R&D Systems, cat. no. 2028-EG-200), polyvinylpyrrolidone (PVP; Sigma, cat. no. PVP360), human chorionic gonadotropin (1,200 IU mL −1 ) (hCG; Sigma, cat. no. C8554), collagenase type IV (1 g mL −1 ; Gibco, cat. no. 17104-019), trypsin-EDTA, 0.25% (Gibco, cat. no. 25200-072), TCM 199(Gibco, cat. no. 11150-059), StemPro-34 SFM (1×), liquid (Gibco, cat. no. 10639-011), and hyaluronidase (500 μg ml −1 , TCI, cat. no. H0164), anti-Ddx4 (polyclonal, 1:500; Abcam), anti-GDF9 (polyclonal, 1:500; Invitrogen), anti-α-tubulin (monoclonal, 1:20, Thermo, cat no. 53-4502-82) fluorescently labeled (Alexa Fluor 488 or 568; Molecular Probes, Eugene, OR, USA), DAPI (molecular probes, Thermo). ..

    Labeling:

    Article Title: Generation of mouse and rat xenogeneic ovaries in vitro for production of mouse oocyte
    Article Snippet: .. The following reagents and chemicals were purchased from respective suppliers: Dulbecco’s modified Eagle’s medium (1×) (DMEM; Gibco, cat. no. 11965-092), phosphate-buffered saline (PBS; Gibco, cat. no. 10010-023), Minimum Essential Medium Eagle, alpha modification (1×) (α-MEM; Gibco, cat. no. 12571–063 and 32571-036), 2-mercaptoethanol (55 mM; Gibco, cat. no. 21985-023), fetal bovine serum (FBS; Gibco, cat. no. 16000-044), GMEM (1×) (Thermo, cat. no. 11710-035), KSR (15%) (Thermo, cat. no. 10828-028), retinoic acid (100 mM; Sigma, cat. no. R2625), GlutaMax (100×) (Thermo, cat. no. 32571-036), non-essential amino acids (100×) (Thermo, cat. no. 11140-050), sodium pyruvate (Gibco, cat. no. 11360-070), Y27632 (Peprotech, cat. no. 1293823), penicillin/streptomycin/L-glutamine (100×) (Gibco, cat. no. 10378-016), Insulin-Transferrin-Selenium (ITS; Thermo, cat. no. 41400-045), ascorbic acid (150 mM; TCI, cat. no. G0394), ICI182,780 (Tocris, cat. no. 1047), follicle-stimulating hormone (FSH; Sigma, cat. no. F4021), epidermal growth factor (EGF; R&D Systems, cat. no. 2028-EG-200), polyvinylpyrrolidone (PVP; Sigma, cat. no. PVP360), human chorionic gonadotropin (1,200 IU mL −1 ) (hCG; Sigma, cat. no. C8554), collagenase type IV (1 g mL −1 ; Gibco, cat. no. 17104-019), trypsin-EDTA, 0.25% (Gibco, cat. no. 25200-072), TCM 199(Gibco, cat. no. 11150-059), StemPro-34 SFM (1×), liquid (Gibco, cat. no. 10639-011), and hyaluronidase (500 μg ml −1 , TCI, cat. no. H0164), anti-Ddx4 (polyclonal, 1:500; Abcam), anti-GDF9 (polyclonal, 1:500; Invitrogen), anti-α-tubulin (monoclonal, 1:20, Thermo, cat no. 53-4502-82) fluorescently labeled (Alexa Fluor 488 or 568; Molecular Probes, Eugene, OR, USA), DAPI (molecular probes, Thermo). ..

    Cell Culture:

    Article Title: Adsorption properties of biologically active derivatives of quaternary ammonium surfactants and their mixtures at aqueous/air interface. I. Equilibrium surface tension, surfactant aggregation and wettability.
    Article Snippet: The adsorption properties of surfactant mixtures containing two types of quaternary derivatives of lysosomotropic substances: alkyl N,N-dimethylalaninates methobromides and alkyl N,N-dimethylglycinates methobromides were studied.. Quantitative and qualitative description of the adsorption process was carried out on the basis of experimentally obtained equilibrium surface tension isotherms.. The results indicated that most of the systems studied revealed synergistic effect both in adsorption and wetting properties.

    Article Title: The Phospholipid Bis(monoacylglycero)Phosphate Confers Antitumour Immunogenicity to Exosomes Secreted by Dendrogenin A, Which Activates Its Biosynthesis in Tumour Cells
    Article Snippet: Red blood cells were depleted using RBC Lysis buffer (BioLegend, France, Cat # 420301) and filtered through a 70 μm filter. .. Then, cells were cultured in 150 mm culture disks (Corning, Cat # 353025) at 3.0×10 6 cells in 20 mL in RPM1 1640 medium supplemented with 2 mM l ‐glutamine, 10% FBS heat‐inactivated at 56°C for 1 h, penicillin and streptomycin (50 units/mL), 1 mM Hepes (Gibco, France, Cat # 15630‐056), 1% non‐essential amino acids (100×) (Gibco, France, Cat # 11140‐050), 50 μmol/L 2‐Mercaptoethanol (Gibco, France, Cat # 31350‐010) (designated “iDC culture medium”), and 20 ng/mL GMCSF (Peprotech, France, Cat # 315‐03‐100UG), at 37°C in a humidified atmosphere with 5% CO2. iDC culture medium containing 20 ng/mL GMCSF was refilled every 3 days. iDC are obtained on day 6 of culture. ..

    Article Title: Predicting drug-induced slowing of conduction and pro-arrhythmia: identifying the 'bad' sodium current blockers
    Article Snippet: .. The cells were cultured in Iscove's modified Dulbecco's medium (IMDM, Gibco, Invitrogen, Ltd., Paisley, UK), which was supplemented with (amounts indicated hereafter are added to 500 mL IMDM): 50 mL fetal bovine serum (Bio-Whittaker, Walkersville, MD, USA), 5 mL non-essential amino acids 100× (Gibco) and 10 mL hypoxanthine/thymidine supplement 50× (Gibco). .. Immediately before use, 400 μg·mL −1 geneticin (Gibco) was added to the medium.

    Centrifugation:

    Article Title: In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix
    Article Snippet: .. The cells were centrifuged at 1600 rpm, 20–25 ̊C for 5 min. To ensure removal of unbound dye, the cell plate was washed with serum free DMEM/F12 and cell centrifugation was carried out at 1600 rpm, 20–25 ̊C for 5 min. Then, DMEM/F12 (Gibco) was enriched with 15% fetal bovine serum (FBS) (Gibco), 1% non-essential amino acids 100 × (Gibco), 1% Glutamax (Gibco), 1% insulin transferrin selenium (ITS) (Gibco) and 1% (5000 units/ml) penicillin/streptomycin (Thermo) and added to cell plate, pipetted, and stained cells observed with fluorescence microscopy. ..

    Staining:

    Article Title: In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix
    Article Snippet: .. The cells were centrifuged at 1600 rpm, 20–25 ̊C for 5 min. To ensure removal of unbound dye, the cell plate was washed with serum free DMEM/F12 and cell centrifugation was carried out at 1600 rpm, 20–25 ̊C for 5 min. Then, DMEM/F12 (Gibco) was enriched with 15% fetal bovine serum (FBS) (Gibco), 1% non-essential amino acids 100 × (Gibco), 1% Glutamax (Gibco), 1% insulin transferrin selenium (ITS) (Gibco) and 1% (5000 units/ml) penicillin/streptomycin (Thermo) and added to cell plate, pipetted, and stained cells observed with fluorescence microscopy. ..

    Fluorescence:

    Article Title: In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix
    Article Snippet: .. The cells were centrifuged at 1600 rpm, 20–25 ̊C for 5 min. To ensure removal of unbound dye, the cell plate was washed with serum free DMEM/F12 and cell centrifugation was carried out at 1600 rpm, 20–25 ̊C for 5 min. Then, DMEM/F12 (Gibco) was enriched with 15% fetal bovine serum (FBS) (Gibco), 1% non-essential amino acids 100 × (Gibco), 1% Glutamax (Gibco), 1% insulin transferrin selenium (ITS) (Gibco) and 1% (5000 units/ml) penicillin/streptomycin (Thermo) and added to cell plate, pipetted, and stained cells observed with fluorescence microscopy. ..

    Microscopy:

    Article Title: In-vivo oogenesis of oogonial and mesenchymal stem cells seeded in transplanted ovarian extracellular matrix
    Article Snippet: .. The cells were centrifuged at 1600 rpm, 20–25 ̊C for 5 min. To ensure removal of unbound dye, the cell plate was washed with serum free DMEM/F12 and cell centrifugation was carried out at 1600 rpm, 20–25 ̊C for 5 min. Then, DMEM/F12 (Gibco) was enriched with 15% fetal bovine serum (FBS) (Gibco), 1% non-essential amino acids 100 × (Gibco), 1% Glutamax (Gibco), 1% insulin transferrin selenium (ITS) (Gibco) and 1% (5000 units/ml) penicillin/streptomycin (Thermo) and added to cell plate, pipetted, and stained cells observed with fluorescence microscopy. ..



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    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy <t>medium</t> (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: <t>100</t> μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.
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    Image Search Results


    (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Journal: Bio-protocol

    Article Title: A Cartilaginous Organoid System Derived From Human Expanded Pluripotent Stem Cells (hEPSCs)

    doi: 10.21769/BioProtoc.5304

    Figure Lengend Snippet: (A) Representative confocal images of human expanded pluripotent stem cell (hEPSC)-derived chondrocytes treated with chondrocyte hypertrophy medium (CHM; control) and CHM plus phentolamine for 14 days, stained with the chondrocyte-specific marker ACAN. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. (B) Relative mRNA expression of COL2A1, COL10A1, and ACAN in pellets treated with CHM (control) and CHM plus phentolamine for 14 days measured by qPCR. Data are shown as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001; ns, non-significant.

    Article Snippet: MEM non-essential amino acids solution (NEAA) (100×) (Thermo Fisher Scientific, Gibco, catalog number: 11140050) 12.

    Techniques: Derivative Assay, Control, Staining, Marker, Expressing

    Journal: Bio-protocol

    Article Title: Isolation and Culture of Primary Pericytes from Mouse

    doi: 10.21769/BioProtoc.5288

    Figure Lengend Snippet:

    Article Snippet: MEM non-essential amino acids (100×) (Thermo Fisher Scientific, catalog number: 11140050) 16.

    Techniques: Concentration Assay